Selected Labels, Stains and Probes
Selected Biological Active Compounds
DAF Compounds - Fluorescent Probes for the Detection of Nitric Oxide
Nitric oxide (NO) is involved in various physiological and pathological processes in the cell and has been implicated in vasodilation, neurotransmission, cytotoxicity, immune response and inflammation. Within cells, nitric oxide synthase (NOS) catalyses the conversion of arginine to citrulline and NO in the presence of molecular oxygen, tetrahydrobiopterin, NADPH and flavin cofactors. Due to the importance of NO, real time detection and quantification of NO is of great interest. However, the extremely short half-life of NO limits the study of its physiological effect in vivo. Therefore NO-sensitive fluorescent probes, such as DAF-2 have been designed and used in real-time imaging of NO.
Zinc Probes for the Detection of Zn2+
Zinc (Zn) is the second most abundant transition metal in the body and it is essential as catalytic, structural and regulatory ion. Zinc ions are involved in homeostasis, immune responses, oxidative stress, apoptosis and aging. Zinc has been proposed to function as a conventional neurotransmitter for the presynaptic neuron and as a transmembrane signal to traverse the postsynaptic neuron. Aberrant zinc metabolism is associated with many neurological diseases including Alzheimer’s disease, Parkinson’s disease and epilepsy. The most suitable technique for in vivo monitoring of zinc has been proven to be fluroescent imaging using fluorescent zinc probes.
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