Liquid Biopsies
DNA Circolante, Nucleosomi, Modifiche Epigenetiche, PBMC
cfDNA Purification Kit
Works with fresh and frozen samples. Process input volumes from 100 μl to 10 ml. Fast, magnetic bead-based purification, compatible with automation. Low elution volumes to concentrate the cell-free DNA. cfDNA Purification Kit
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Nu.Q Assays: Circulating cf-nucleosomes
To determine the total levels of intact cell free circulating nucleosomes in samples: Nucleosomes are released into plasma following cell death and apoptosis. Nucleosomes are detected at increased rates in cancer. Nucleosomes are more stable structures in circulation compared to cfDNA alone and can be detected by ELISA in serum and plasma. Nu.QTM H3 Assay Kit - Nu.QTM H3.1 Assay Kit
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Histone H3 Modification ELISAs
Screening extracts for specific histone modifications is also a simple way to assess cell health and compound effects.
DNA methylation analysis - Kits
Highly sensitive, can detect a little as 0.5% methylation. Suitable for use with cell- or tissue-derived human genomic DNA
The MeDIP Kit is designed to immunoprecipitate and enrich single-stranded DNA (ssDNA) fragments containing 5-methylcytosine (5-mC). The MeDIP Kit contains a monoclonal 5-methylcytosine antibody, a bridging antibody, and the necessary buffers to perform methylated DNA immunoprecipitation. The high specificity and affinity of the antibody enables separation of 5-mC DNA from DNA containing 5-hmC and other DNA methylation variants using single-stranded input DNA.
The hMeDIP Kit is designed to immunoprecipitate and enrich for DNA fragments containing 5-hydroxymethylcytosine (5-hmC). The hMeDIP Kit contains a highly specific purified 5-hmC antibody and the necessary buffers to perform methylated DNA immunoprecipitation (MeDIP). The affinity of the antibody enables separation of 5-hmC DNA from 5-mC DNA. Additionally, the antibody works to efficiently immunoprecipitate either double-stranded or single-stranded input DNA.affinity of the antibody enables separation of 5-mC DNA from DNA containing 5-hmC and other DNA methylation variants using single-stranded input DNA.
A fast magnetic assay capable of efficiently isolating methylated CpG islands from fragmented genomic DNA. MethylCollector Ultra is based on the Methylated CpG Island Recovery Assay (MIRA) which uses a MBD2b/MBD3L1 protein complex for improved enrichment of CpG dinucleotides.
ChIP-IT® PBMC Kit
Perform ChIP on difficult-to-lyse PBMCs. Active Motif’s ChIP-IT® PBMC Kit overcomes the challenges of generating quality chromatin for use in ChIP from peripheral blood mononuclear cells (PBMCs). PBMCs, including lymphocytes (T cells, B cells and NK cells), monocytes and dendritic cells, constitute a critical component of the peripheral immune system and are often used in immunology, cancer and infectious disease research. ChIP-IT® PBMC Kit
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