Elispot
ELISpot and FluoroSpot
Human IgG SARS-CoV-2 RBD ELISpot PLUS (HRP)
3850-4HPW-R1-1
ELISpotPLUS for enumeration of cells IgG antibodies specific for SARS-CoV-2 RBD
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for enumeration of B cells secreting IgG antibodies specific for the SARS-CoV-2 Receptor Binding Domain (RBD). Enumeration of all cells secreting IgG (total IgG) can also be made.
The kit includes one ELISpot strip plate pre-coated with monoclonal anti-human IgG antibodies, recombinant RBD-WASP (recombinant RBD with site specific peptide tag WASP), biotinylated detection mAb, anti-WASP-HRP, Streptavidin-HRP, polyclonal activators, and ready-to-use TMB substrate. Pre-coated plates reduce assay time and minimize variability.
For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).