Elispot
ELISpot and FluoroSpot – methods for SARS-CoV-2 vaccine development
3850-4HPW-R1-1
ELISpotPLUS for enumeration of cells IgG antibodies specific for SARS-CoV-2 RBD
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for enumeration of B cells secreting IgG antibodies specific for the SARS-CoV-2 Receptor Binding Domain (RBD). Enumeration of all cells secreting IgG (total IgG) can also be made.
The kit includes one ELISpot strip plate pre-coated with monoclonal anti-human IgG antibodies, recombinant RBD-WASP (recombinant RBD with site specific peptide tag WASP), biotinylated detection mAb, anti-WASP-HRP, Streptavidin-HRP, polyclonal activators, and ready-to-use TMB substrate. Pre-coated plates reduce assay time and minimize variability.
For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
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MabTech Elispot
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Find 1 cell in 100,000 with ELISpot
ELISpot is a sensitive assay used to quantify cytokine- or immunoglobulin-secreting cells at the single-cell level.
• Sensitive detection of antigen-specific cytokine responses
• Investigation of vaccine efficacy and evaluation of novel vaccine adjuvants
• Characterization of cytotoxic T-cell activity by IFN-γ, granzyme B, and perforin analysis
• Quantification of antigen-specific memory B cells and vaccine-induced antibody responses
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Mabtech FluoroSpot
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Discover more with FluoroSpot
FluoroSpot combines the sensitivity of ELISpot with the capacity to analyze secretion of several analytes simultaneously.
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