THUNDER™ Total GAPDH TR-FRET Cell Signaling Assay Kit
CAY-500249-9600 9600 wells
Synonyms: Glyceraldehyde-3-phosphate dehydrogenase
Data Sheet: 
SDS:
SDS:
MW
0
Shipment
-80
Long Term Storage
-80
Shelf Life
180
Description
The Total GAPDH assay kit is a homogeneous time-resolved Förster resonance energy transfer (TR-FRET) sandwich immunoassay. The THUNDER™ Cell Signaling assay workflow consists of 3 steps. Following cell treatment, cells are first lysed with the specific Lysis Buffer provided in the kit. Then Total GAPDH in the cell lysates is detected with a pair of fluorophore-labeled antibodies in a simple "add-incubate-measure" format(singlestep reagent addition; no wash steps). One antibody is labeled with a donor fluorophore (Europium chelate; Eu-Ab1) and the second with a far-red acceptor fluorophore (FR-Ab2). The binding of the two labeled antibodies to distinct epitopes on the target protein takes place in solution and brings the two dyes into close proximity. Excitation of the donor Europium chelate molecules with a flash lamp (320 or 340 nm) or a laser (337 nm) triggers a FRET from the donor to the acceptor molecules, which in turn emit a TR-FRET signal at 665 nm. Residual energy from the Eu chelate generates light at 615 nm. The signal at 665 nm is proportional to the concentration of Total GAPDH in the cell lysate. Data can be expressed as either the signal at 665 nm or the 665 nm/615 nm ratio.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
