Involucrin Antibody
NSJ-V2164-100UG 100 ug
Data Sheet: 
Reactivity
Human
Applications
IHC-P
Source / Host
Mouse
Immunogen
Purified Involucrin from human keratinocytes was used as the immunogen for this antibody.
Clone
SY5
Purity
Protein G affinity chromatography
Long Term Storage
Store the Involucrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Description
This antibody recognizes a protein of 66-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
