CD125 (IL-5RA): IL-5 [Biotinylated] Inhibitor Screening  Chemiluminescence Assay Kit

  BPS-82965 96 Reactions

Data Sheet:    

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Human  

Study and screen compounds that inhibit the binding of CD125 to IL-5 for drug discovery in high throughput screening (HTS) applications.  

-80°C  

Schleich F., et al., 2023 Eur Respir Rev 32(168): 220193.  

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Description

 

The CD125 (IL-5RA): IL-5 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is an ELISA-based assay designed to measure the binding between CD125 (also known as IL-5RA or Interleukin 5 receptor subunit alpha) and IL-5 protein for screening and profiling applications. This kit comes with enough purified CD125 (amino acids 21-342) and biotinylated IL-5 (amino acids 20-134(end)), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. Figure 1. CD125 (IL-5RA): IL-5 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit schematic. A 96-well plate is coated with CD125 protein. After coating and blocking, biotinylated IL-5 is added in an optimized assay buffer. Next, unbound biotinylated IL-5 is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of IL-5 binding to CD125. Need us to run inhibitor screens or profile your compounds that block the binding of IL-5 to IL-5RA? Check out our Immunotherapy Biochemical Screening Services. - Study and screen compounds that inhibit the binding of CD125 to IL-5 for drug discovery in high throughput screening (HTS) applications. - Avoid freeze/thaw cycles.

 

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