BRD2 bromodomain 2 (human, recombinant)

  CAY-11070-1 1 mg

Data Sheet:     SDS:    

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50 mM Tris, pH 7.5, with 500 mM sodium chloride, 5% glycerol, and 5 mM β-mercaptoethanol  

≥95% estimated by SDS-PAGE  

0  

-80  

-80  

730  

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Description

 

Bromodomain-containing 2 (BRD2) is a transcriptional regulator that is a member of the bromodomain and extra-terminal (BET) family.{33144} It is ubiquitously expressed and localizes to the nucleus. BRD2 is composed of two N-terminal bromodomains (BD1 and BD2) that bind acetylated lysine on histones, serving to couple histone acetylation marks to the transcriptional regulation of target promoters, and an extra-terminal domain that mediates chromatin interactions.{33144,55056} BRD2 associates with transcription effector and regulator proteins, including RNA polymerase II, histone acetylases and deacetylases, and transcriptional co-activators and co-repressors to form a transcription complex that regulates the expression of genes involved in inflammation and cell proliferation.{33144,55055} BRD2 also binds to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) envelope (E) protein, a transmembrane protein involved in CoV virion assembly and pathogenesis of the related virus, SARS-CoV.{55024,53462,49521} The BD2 bromodomain of BRD2 contains four α-helices that form the binding site for the acetylated histone H4 tail.{19961} Point mutations of valine 329 (V329A) or asparagine (N382A) in the BD2 bromodomain eliminate BRD2 binding to acetylated histone H4 lysine 12 (H4K12Ac), and a point mutation of tyrosine 427 (Y427F) abolishes the association between BRD2 and STAT3.{55111,55093} Cayman’s BRD2 bromodomain 2 (human, recombinant) protein can be used for ELISA, Western blot (WB), and binding assay applications.

 

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