Anti-Estrogen Receptor α (Tyr-537), Phosphospecific Antibody

  ABI-EM5451 100 µL

Data Sheet:    

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Chicken, Human, Mouse, Rat, and Xenopus  

ELISA, WB  

M545  

Blue Ice  

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.  

After date of receipt, stable for at least 1 year at -20°C.  

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Description

 

Our Estrogen Receptor α (Tyr-537) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Estrogen Receptor α (Tyr-537) and is antigen affinity purified. It is great for use in WB. - Estrogen receptor α (ERα) is a member of the steroid receptor superfamily and its structure includes an N-terminal ligand-independent transactivation domain (AF-1), a highly conserved DNA binding domain, and a C-terminal ligand-dependent transactivation domain (AF-2). AF-1 and AF-2 activate transcription independently and synergistically, and act in a promoter- and cell-specific manner. Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity. Ser-104, Ser-106, Ser-118, and Ser-167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. In addition to these sites, phosphorylation of Tyr-537 has been implicated in maximal hormone binding, dimerization, and transcriptional activity. Tyr-537, located in the AF-2 domain, is phosphorylated by c-Src leading to nuclear export of ERα and degradation. Thus, a variety of phosphorylation events control ERα activity. -

 

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