Anti-Doublecortin (DCX) Antibody
ABI-M-1648-100 100 µg
Synonyms: Doublin, Lissencephalin-X, DBCN and Lis-X
Reactivity
Bovine, Human, Mouse, and Rat
Applications
ICC, WB
Clone
30
Shipment
25°C (ambient)
Long Term Storage
Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a hi
Shelf Life
12 months after date of receipt (unopened vial).
Description
Our Anti-Doublecortin (DCX) mouse monoclonal primary antibody detects bovine, human, mouse, and rat Doublecortin (DCX), and is IgG. It is validated for use in ICC, WB. - Doublecortin (DCX, also known as Doublin, Lissencephalin-X, DBCN and Lis-X) was originally discovered since defects in the gene encoding it are causative of X-linked lissencephaly, a rare group of brain malformations resulting in a smooth cerebral cortex caused by aberrant neuronal migration during development (1,2). The name Doublecortin comes from the unusual layering of the cortex in this form of lissencephaly, which appears to have a second deep cortical layer of neurons. This layer consists of neurons which did not migrate from the subventricular zone to the normal cortical layer. Patients with this defect suffer from seizures and mental retardation. Four proteins encoded by the DCX produce bands of about 35 kDa and 45 kDa on Western blots. The 45 kDa form is known as Lis-XA while the smaller forms are generated by alternate transcription, are all missing the first 81 amino acids of Lis-XA, and are referred to as Lis-XB, Lis-XC, Lis-XD. There are minor amino acid sequence differences between these three smaller isoforms. All of these proteins contain two so-called Doublecortin domains, each about 90 amino acids long, which are believed to function in binding to microtubules, a C-terminal serine and proline rich region which may become phosphorylated in vivo. DCX is expressed very early in neuronal development, as neuroblasts become post-mitotic, but is lost as neurons mature. Developing neurons start to lose DCX expression about the time that they begin to express NeuN. Antibodies to DCX can be used to see if neurogenesis is taking place. -
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
