A-431 nuclear extract (EGF treated)
V13-36003 200 µg
Synonyms: A-431, A431, EGF, treated, stimulated, nuclear extract, nuclear, extract, A-431 nuclear extract, nuclear, extract, 431, nuclear extract, nuclear, extract, lysate, nuclear lysate, A431 nuclear extract, A-431 cell extract, A-431 cell nuclear extract, A431 n
Data Sheet: 
Shipment
Dry Ice
Short Term Storage
-80C
Long Term Storage
To ensure stability, extracts should be stored at -80ºC.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC. This eliminates repeated freeze/thaw cycles.
Description
Active Motif's A-431 nuclear extract (EGF treated) was prepared from cell cultures of the human epidermoid carcinoma A-431 cell line. These cells are characterized by abnormally high levels of expression of epidermal growth factor receptor (EGFR), approximately 3 x 10E6/cell, on the cell surface and exhibits a biphasic response to varying concentrations of epidermal growth factor (EGF). Specifically, lower (pmol) concentrations of EGF stimulates cell growth while higher (nmol) concentrations inhibits cell growth and induces terminal differentiation. Therefore, these cells are commonly used as a positive control for EGFR expression and in studies of EGFR downstream signaling cascades. These cells are also highly responsive to mitogenic stimuli and are naturally devoid of p53 and so are also frequently used in cancer and cell cycle-associated studies. This extract is for analysis of EGFR expression and downstream signaling cascades.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
