5hmC Profiling Kit
V13-55023 24 rxns
Synonyms: Hydroxymethyl Collectory, Hydroxymethyl, Hydroxy, methylation, DNA methylation, 5-hmC, 5 hmc, OH-CH2, 5-mC, 5 mC, affinity enrichment, b-glucosyltransferase, beta glucosyltransferase, glucosyl, glucosylation
Shipment
Dry Ice
Short Term Storage
-20C
Long Term Storage
Storage conditions range from room temperature to -20°C. Please refer to the product manual for proper storage temperatures of each component.
Description
Based on the patented 5hmC-Seal technology the 5hmC Profiling Kit protocol is designed to detect and capture DNA fragments containing 5-hydroxymethylcytosine (5hmC) DNA methylation for analysis by real-time PCR or NGS sequencing. By utilizing a beta-glucosyltransferase enzyme a modified glucose moiety is selectively transferred to 5hmC residues in double-stranded DNA. The modified glucose is then used to chemically attach a biotin conjugate for capture and enrichment of 5hmC containing fragments with streptavidin magnetic beads. All purification steps are done with convenient SPRI beads.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
