3CL Protease (B.1.1.529, Omicron Variant) (SARS-CoV-2) Assay Kit
BPS-78350-1 96 rxns.
Synonyms: main protease kit, Mpro, SARS-CoV-2 3CL Protease kit, nsp5, 78350-1, 78350-2
Data Sheet: 
Reactivity
SARS-CoV-2
Applications
Study enzyme kinetics and screen small molecular inhibitors for drug discovery and High Throughput (HTS) applications.
Shipment
-80°C (dry ice)
Literature References
1. Morse, J.S., et al., 2020 Chem.Bio.Chem. 21: 730 - 738.
2. Chi-Pang, C., et al., 2011 PLoS ONE 6(11): e27228.
2. Chi-Pang, C., et al., 2011 PLoS ONE 6(11): e27228.
Description
The 3CL Protease (B.1.1.529, Omicron Variant) (SARS-CoV-2) Assay Kit is designed to measure the activity of P132H mutated, Omicron variant 3CL Protease for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.
The 3CL Protease Substrate is an internally quenched 14-mer fluorogenic (FRET) peptide (DABCYL-KTSAVLQSGFRKME-EDANS). When the donor (EDANS) and acceptor (DABCYL) fluorophores are in close proximity, the energy emitted from EDANS is quenched by DABCYL (intact substrate). Upon proteolysis by 3CL, the peptide substrate is cleaved between glutamine and serine to generate the highly fluorescent peptide fragment (SGFRKME-EDANS). The fluorescence intensity increases proportionally to the activity of 3CL. More information on the substrate, including MW and structure, can be found on our website (BPS Bioscience, #79952).
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
