293 nuclear extract
V13-36033 200 µg
Synonyms: 293, nuclear extract, nuclear, extract, HEK, HEK 293, 293 nuclear extract, 293 cell extract, 293 cell nuclear extract, 293 nuclear cell extract, human embryonic kidney, embryonic kidney, splicing, pre-mRNA splicing, pre-mRNA, mRNA, mRNA splicing, mRNA pro
Data Sheet: 
Shipment
Dry Ice
Short Term Storage
-80C
Long Term Storage
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC. This eliminates repeated freeze/thaw cycles.
Description
Active Motif's 293 nuclear extract was prepared from cell cultures of the Human Embryonic Kidney (HEK) 293 cell line. This cell line was originally derived from transformation of normal human embryonic tissue cultures with a sheared DNA fragment of the left arm of the adenoviral 5 viral genome. These cells are commonly used in research due to their ease of growth and transfectability. However, since the cellular origin of these hypotriploid cells is poorly understood, they are not recommended for use as an in vitro model for kidney cell function. 293 nuclear extract contains all the basal transcriptional and mRNA splicing components and serves as an ideal cell-free platform for analysis of transcription and pre-mRNA processing mechanisms. This extract is used in in vitro transcription, protein-protein and protein-DNA/RNA interactions.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
