Vinci-Biochem Srl
Via Ponte di Bagnolo, 10
50059 Vinci (Firenze) Italy
vb@vincibiochem.it
Tel:+39 0571 568 147 -
 0571 568 135
Fax:+39 0571 568132 - P. IVA 05706610481

Autoimmune Diseases Research 


 

Citrullination Research Tools
Citrullination Research Tools Cayman
AdipoGen ELISA Kits
ELISA Kits
AdipoGen Life Sciences
BAFF human ELISA Kit hypersensitive, Obesity/Type 2 Diabetes Markers, Insulin Resistance Markers, Inflammatory Diseases Biomarkers: Rheumatoid Arthritis, Neuroinflammation Markers. Autoimmune Diseases Biomarkers. 
       
Cayman Neutrophil Biology
Neutrophil Biology Currents - Cayman
NET Quantification: NETosis Assay Kit
Citrullinated Histone H3 ELISA
Antibodies for NET Research
Neutrophil Isolation and Function
PAD, Peptidylarginine deiminases Reagents
Hycult Complemento
Complement and collectins
ELISA kits, Antibodies and proteins for coagulation factors and complement proteins
C1q, C3a, C3c, MASP
       
Cayman NET Technical Brief
NET Technical Brief - Cayman 
TECHNICAL BRIEF
Quantify Neutrophil
Extracellular Traps by ELISA
Alpha Autoimmunity Research ELISA kits
Autoimmunity Research ELISA Kits - Alpha Diagnostics
Bovine, Human, Monkey, Mouse, Rabbit, Rat and Sheep ELISA Kits
       
Assay Kits Cayman
Assay Kits Cayman
Immunoassays to Mass Spectrometry
Kits by Research Area
Cancer
Cardiovascular Disease
Cell Biology
Eicosanoids & Lipids
Epigenetics
Immunology & Inflammation
Metabolism
Neuroscience
Nitric Oxide & Oxidative Injury
Toxicology
Kit Information 
Sample Preparation & Screening Libraries

 

 
 

    

 
NETosis Assay Kit 601010 
NETosis Assay Kit
Features
•Induce and detect NET formation in vitro
•Elastase-based readout
•Non-dsDNA readout eliminates false positives from DNA
•Adaptable to multiple species
•Neutrophil (mouse) Isolation Kit (Item No. 601070) available to isolate mouse neutrophils from peritoneal lavage or bone marrow
 
Cayman's NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman's NETosis Assay Kit does not depend upon the DNA component of neutrophil extracellular traps (NETs), as DNA release can occur independently of NETosis. In this kit, primary neutrophils are stimulated to release NETs with either PMA or a calcium ionophore (both included). Unbound neutrophil elastase is washed away following NET generation. Following digest of NET DNA by S7 nuclease, the supernatant containing neutrophil elastase is added to a substrate, which is selectively cleaved by elastase to yield a 4-nitroaniline product that adsorbs light at 405 nm. Enough reagents are provided to test 24 sample conditions for NET production, with analysis in duplicate.

 





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    Vinci-Biochem Srl
    Via Ponte di Bagnolo, 10
    50059 Vinci (Firenze) Italy
    vb@vincibiochem.it
    Tel:+39 0571 568147
    Fax:+39 0571 568132
    P. IVA 05706610481