Cyclin B1 Antibody / CCNB1
NSJ-V2946IHC-7ML 7 ml
Data Sheet: 
Reactivity
Human
Applications
IHC-P
Source / Host
Mouse
Immunogen
Recombinant human full-length protein was used as the immunogen for the Cyclin B1 antibody.
Clone
CCNB1/1098
Purity
Protein G affinity chromatography
Long Term Storage
Store the Cyclin B1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Description
It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
ESCLUSIVAMENTE PER USO DI RICERCA (RUO) e non per uso terapeutico o diagnostico su uomini o animali. Il prodotto NON è un Dispositivo Medico o un Diagnostico in Vitro.
PRODUCT FOR RESEARCH USE ONLY (RUO) and not for therapeutic or diagnostic use on humans or animals. The product is NOT a Medical Device or an In-Vitro Diagnostic (IVD).
